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. 1999 Mar 1;189(5):767–778. doi: 10.1084/jem.189.5.767

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Presentation of the MAGE-3 antigen by dendritic cells incubated with purified protein or cell lysates. (A) HLA-DR13 dendritic cells were cultured for 24 h with different concentrations of MAGE-3 protein. The cells were washed, then incubated with 2,500 cells per well of clone 37. IFN-γ production was measured after 20 h by ELISA. The results shown represent the average of triplicate cultures. (B) 293-EBNA cells (5 × 10⁵ cells per well) were transfected with different doses of pCEP4-MAGE-3 mixed with Lipofectamine^®. 24 h after transfection, the transfected cells were lysed by freeze–thawing. HLA-DR13 dendritic cells (10⁵ cells per well) were cultured with lysates at the equivalent of 5 293-EBNA cells per dendritic cell for 24 h. The experiment was pursued as in A.

Presentation of the MAGE-3 antigen by dendritic cells incubated with purified protein or cell lysates. (A) HLA-DR13 dendritic cells were cultured for 24 h with different concentrations of MAGE-3 protein. The cells were washed, then incubated with 2,500 cells per well of clone 37. IFN-γ production was measured after 20 h by ELISA. The results shown represent the average of triplicate cultures. (B) 293-EBNA cells (5 × 10⁵ cells per well) were transfected with different doses of pCEP4-MAGE-3 mixed with Lipofectamine^®. 24 h after transfection, the transfected cells were lysed by freeze–thawing. HLA-DR13 dendritic cells (10⁵ cells per well) were cultured with lysates at the equivalent of 5 293-EBNA cells per dendritic cell for 24 h. The experiment was pursued as in A.