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. 1999 Mar 1;189(5):767–778. doi: 10.1084/jem.189.5.767

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Recognition by clone 37 of cells expressing an Ii MAGE-3 construct. (A) MZ2-MEL.43 melanoma cells express both HLA-DR13 and MAGE-3. Cells were pulsed for 2 h with 5 μg/ml of peptide MAGE-3_115–130 and washed, or transduced with a retroviral construct encoding Ii.MAGE-3. (B) MZ2-EBV cells express HLA-DR13. As a positive control for stimulating cells, we incubated them for 20 h with 20 μg/ml of protein MAGE-3. MZ2-EBV cells were transduced with a retroviral construct encoding MAGE-3 alone or Ii.MAGE-3. Tumor cells (10⁴) and EBV-B cells (5 × 10³) were distributed in microwells, and 2,500 cells of clone 37 were added. IFN-γ production was measured after 20 h of coculture by ELISA. The results shown represent the average of triplicate cultures.