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. 1999 Mar 1;189(5):757–766. doi: 10.1084/jem.189.5.757

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Purification of the 1558-mel Ag by hydrophobic interaction chromatography. Individual eluate fractions (5 × 10⁷ cell equivalents/ well) were pulsed onto APCs for T cell recognition. SDS-PAGE (silver stain) shows that bioactive fractions 4 and 6 appear to contain a single protein band of ∼30 kD. The protein in fraction 6 was sequenced via mass spectrometric analysis, identifying it as TPI. This was confirmed by NH₂-terminal Edman degradation sequencing of fraction 4.