Table I.
Recognition of Mutated TPI by CD4+ TIL 1558
| Stimulatorcell lysate |
Transfected gene |
cDNA sequence,nucleotide 450 |
GM-CSF | |||
|---|---|---|---|---|---|---|
| pg/ml | ||||||
| 1558-mel | None | T, C | 6,315 | |||
| 1558-EBV | None | C | <8 | |||
| COS-7 | TPI-1B | T | 3,485 | |||
| COS-7 | TPI-5B | T | 8,310 | |||
| COS-7 | TPI-11G | T | 6,340 | |||
| COS-7 | TPI-4B | C | 8 | |||
| COS-7 | TPI-12A | C | 11 | |||
| COS-7 | TPI-12F | C | 11 | |||
| COS-7 | β-actin | n.a. | <8 |
COS-7 cells were transfected for 4 d with the indicated genes. Cell lysates were then incubated with THP-1 monocytic leukemia cells for 20 h, after which TIL were added for 20 h. GM-CSF secretion by TIL was measured by ELISA. n.a., not applicable.