Table II.

Determining the PI Anchor Residue for TPI_mut 23–37

TPImut residues		Sequence		GM-CSF
				pg/ml
23–37		G E L I G I L N A A K V P A D		25,390
24–37		E L I G I L N A A K V P A D		28,460
25–37		L I G I L N A A K V P A D		11,750
26–38		I G I L N A A K V P A D T		7,210
27–39		G I L N A A K V P A D T E		38
28–40		I L N A A K V P A D T E V		22
29–41		L N A A K V P A D T E V V		34
20–34		Q S L G E L I G I L N A A K V		5,960
20–33		Q S L G E L I G I L N A A K		35
20–32		Q S L G E L I G I L N A A		25
20–31		Q S L G E L I G I L N A		24

THP-1 cells pretreated with IFN-γ were pulsed overnight with the indicated peptides at 100 nM, then T cells were added for 20 h. GM-CSF secretion by T cells was measured by ELISA. Background secretion of T cells plus THP-1 without peptide = 28 pg/ml.