Figure 5.
Localization of PbCTRP. Parasites were collected 10 and 22 h after exflagellation, purified by erythrocyte lysis, and fixed in acetone for 2 min. Specific antigen was revealed using an antiserum against recombinant PbCTRP as described in Materials and Methods. (a) 10 h after exflagellation; (b) 22 h after exflagellation; (c) 22 h after exflagellation, stained using nonimmune rabbit serum (as control). Arrows indicate mature ookinetes in control. Original magnifications: ×200.