Figure 3.

IFN-γ production by DC subpopulations. (A) Isolated splenic DCs were stained with anti-CD11c–FITC, anti-CD8α–PE, and anti-CD86– biotin, followed by streptavidin–Red 670, and subjected to cell sorting to purify CD8α⁻CD11c⁺CD86⁺ (myeloid) DCs and CD8α⁺CD11c⁺CD86⁺ (lymphoid) DCs. Purity of CD11c⁺CD86⁺ whole DCs, CD8α⁻ DCs and CD8α⁺ DCs were 98, 95, and 90%, respectively. (B) Whole DCs, CD8α⁻CD11c⁺CD86⁺ DCs, and CD8α⁺ CD11c⁺CD86⁺ DCs (5 × 10⁴) were cultured for 3 d in the presence of 1 ng/ml IL-12, and the amounts of IFN-γ in culture supernatants were measured by ELISA. No difference in viability was observed between CD8α⁺ and CD8α⁻ DCs.