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. 1999 Jun 21;189(12):1981–1986. doi: 10.1084/jem.189.12.1981

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Detection of intracellular IFN-γ in IL-12–stimulated DCs. Purified DCs were cultured in the presence of 1 ng IL-12 for 3 d. After surface staining with FITC-conjugated mAb against CD11c, cells were fixed and permeabilized. Cells were then incubated with rabbit polyclonal Ab against IFN-γ (A and B) and normal rabbit serum (E and F). Freshly isolated DCs were also incubated with rabbit polyclonal Ab against IL-12R (C and D). Samples were further stained with Rhodamine-conjugated goat anti-rabbit IgG. Bars, 10 μm.