Figure 1.

TCR antagonism in 58CD8α/β T cell hybridomas expressing the Vα2Vβ5 (OT-I) TCR. T cell hybridomas were stimulated with their antigen, SIINFEKL, and the effect of the antagonist (E1, P7) and control (K4) peptides on IL-2 response (A) and TCR downregulation (B) was measured. The arrow highlights the data showing that, in the absence of SIINFEKL, neither the antagonists E1 and P7, nor the control peptide K4 induced TCR internalization. The IL-2 response (A) was determined after 25 h of stimulation, while the TCR downregulation (B) was analyzed after 3 h. Data are expressed as mean of triplicates in A or of duplicates in B. This experiment is representative of eight separate experiments.