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. 1999 Jan 18;189(2):423–434. doi: 10.1084/jem.189.2.423

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Absolute donor cell numbers recovered from lung lavage, spleen, and TBLN on days 1, 3, 5, and 7 after influenza virus infection and cell transfer. 10⁷ Tc1 and Tc2 effectors expressing Thy 1.2 were adoptively transferred into influenza-infected Thy 1.1 BALB/c hosts as described in Fig. 3. To assess absolute donor cell numbers in the different organs, total cell counts from the isolated organs were performed and were multiplied by the percentages of donor cells determined by FACS^® as shown in Fig. 3. Here, three animals per group were analyzed and the average donor cell numbers ± SE isolated from lung lavage (a), TBLN (b), and spleen (c) are shown. It should be noted that the adoptively transferred cells were injected at 4 PM on day 0 and the day 1 mice were killed at 7 AM, an interval of only 15 h.