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. 1999 Jan 18;189(2):309–318. doi: 10.1084/jem.189.2.309

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PIR expression by thymic and splenic dendritic cells. (Top) Thy-1⁺ T and B220⁺ B cells were depleted from thymic cell suspensions by complement-mediated lysis, and the remaining cells were stained with the indicated mAbs. The CD11c⁺/CD19⁻ cells, which comprised ∼1% of the initial MNC population, were analyzed for the expression of PIR and other cell surface markers (MHC I-A^d, Mac-1, CD8, and FcγRII/ III). (Bottom) Splenocytes were stained similarly, and the CD11c⁺/CD19⁻ cells analyzed for other cell surface antigens.