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. 1999 Jan 18;189(2):289–300. doi: 10.1084/jem.189.2.289

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Flow cytometric analysis of γ/δ T cell populations in E2A-deficient mice. Flow cytometric analysis of thymus and lymph node (LN) cells from a 6-wk-old E2A-deficient mouse and a heterozygous littermate (A). Cells were stained with anti-CD4^PE, anti-CD8^PE, and anti–γ/δ TCR^FITC. The number shown above each FACS^® profile represents the total number of cells isolated from that tissue. The percentages of γ/δ cells are indicated. The total number of γ/δ cells is decreased 10–20-fold in the thymus and lymph nodes of E2A-deficient mice. (B) Intestinal T cells from an E2A-deficient mouse and a heterozygous littermate were stained with anti–α/β TCR^cy-chrome and anti–γ/δ TCR^biotin (streptavidin-PE). The total number of intestinal lymphocytes (top) is decreased approximately sevenfold in the E2A-deficient mice (3 vs. 23%). Bottom panels show the α/β versus γ/δ staining of the lymphocytes gated in the top panels with the percentages of each population indicated. (C) Cells isolated from the skin of an E2A-deficient mouse and a heterozygous littermate were stained with anti-Thy1.1^biotin (streptavidin–Red 670), anti-CD3^PE, and anti-Vγ3^FITC. T cells were gated on the basis of forward scatter and Thy1.1 expression (top), and the gated cells were analyzed for the expression of CD3 and Vγ3 (bottom).

Flow cytometric analysis of γ/δ T cell populations in E2A-deficient mice. Flow cytometric analysis of thymus and lymph node (LN) cells from a 6-wk-old E2A-deficient mouse and a heterozygous littermate (A). Cells were stained with anti-CD4^PE, anti-CD8^PE, and anti–γ/δ TCR^FITC. The number shown above each FACS^® profile represents the total number of cells isolated from that tissue. The percentages of γ/δ cells are indicated. The total number of γ/δ cells is decreased 10–20-fold in the thymus and lymph nodes of E2A-deficient mice. (B) Intestinal T cells from an E2A-deficient mouse and a heterozygous littermate were stained with anti–α/β TCR^cy-chrome and anti–γ/δ TCR^biotin (streptavidin-PE). The total number of intestinal lymphocytes (top) is decreased approximately sevenfold in the E2A-deficient mice (3 vs. 23%). Bottom panels show the α/β versus γ/δ staining of the lymphocytes gated in the top panels with the percentages of each population indicated. (C) Cells isolated from the skin of an E2A-deficient mouse and a heterozygous littermate were stained with anti-Thy1.1^biotin (streptavidin–Red 670), anti-CD3^PE, and anti-Vγ3^FITC. T cells were gated on the basis of forward scatter and Thy1.1 expression (top), and the gated cells were analyzed for the expression of CD3 and Vγ3 (bottom).