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. 1999 Jan 18;189(2):289–300. doi: 10.1084/jem.189.2.289

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Differential usage of the Vγ and Vδ gene segments in E2A-deficient mice. (A and B) PCR analysis of total thymus DNA from adult (6-wk-old) E2A-deficient mice and their heterozygous littermates to determine the relative level of Vγ (A) and Vδ (B) gene rearrangements. Forward primers were specific for each V region. The Jγ1 reverse primer used recognizes all Jγ gene segments, whereas the reverse Jδ primers are specific for either Jδ1 or Jδ2. The δ PCRs were performed with both reverse primers, and the results were identical. A control reaction was performed with p53 primers. Shown are three independent knockouts (designated a, b, and c) and their heterozygous littermates.

Differential usage of the Vγ and Vδ gene segments in E2A-deficient mice. (A and B) PCR analysis of total thymus DNA from adult (6-wk-old) E2A-deficient mice and their heterozygous littermates to determine the relative level of Vγ (A) and Vδ (B) gene rearrangements. Forward primers were specific for each V region. The Jγ1 reverse primer used recognizes all Jγ gene segments, whereas the reverse Jδ primers are specific for either Jδ1 or Jδ2. The δ PCRs were performed with both reverse primers, and the results were identical. A control reaction was performed with p53 primers. Shown are three independent knockouts (designated a, b, and c) and their heterozygous littermates.