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. 1999 Jan 18;189(2):289–300. doi: 10.1084/jem.189.2.289

Figure 4.

Figure 4

Figure 4

PCR analysis of V gene rearrangements in E2A-deficient fetal thymus DNAs. Vγ (A) and Vδ (B) gene rearrangements from E19 fetal thymus DNAs. All fetal thymus DNA samples shown are from the same litter. (C) PCR analysis of Vδ5 and Vδ1 gene rearrangements from two independent adult (6-wk-old) E2A heterozygous mice and their wild-type littermates. Vδ5 rearrangements from five E19 fetal thymocyte DNAs are shown for comparison. The number of PCR cycles used for the adult Vδ1 rearrangements in C was increased (compared with Fig. 3 B) in order to enhance the signal from the heterozygotes.