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. 1999 Jan 18;189(2):289–300. doi: 10.1084/jem.189.2.289

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Detection of δ gene intermediate rearrangement products in E2A-deficient mice and their heterozygous littermates. (A) Schematic diagram of the δ locus showing the relative location of the forward and reverse primers used to detect V-D intermediate rearrangement products. A fully rearranged V-D-J allele results in the deletion of the reverse primer, which is located 3′ of Dδ2. As a result, only the intermediate V-D rearrangements can be PCR amplified. (B) Diagram showing the location of the PCR primers used in the detection of D-J intermediates. Rearrangement of any V region to Dδ2 results in the deletion of the forward PCR primer. Thus, only D-J intermediates can be PCR amplified. (C) Vδ1-Dδ2, Vδ5-Dδ2, and Dδ2-Jδ1 PCR analysis of total thymus DNA from three independent 6-wk-old E2A-deficient mice (designated a, b, and c) and their heterozygous littermates.

Detection of δ gene intermediate rearrangement products in E2A-deficient mice and their heterozygous littermates. (A) Schematic diagram of the δ locus showing the relative location of the forward and reverse primers used to detect V-D intermediate rearrangement products. A fully rearranged V-D-J allele results in the deletion of the reverse primer, which is located 3′ of Dδ2. As a result, only the intermediate V-D rearrangements can be PCR amplified. (B) Diagram showing the location of the PCR primers used in the detection of D-J intermediates. Rearrangement of any V region to Dδ2 results in the deletion of the forward PCR primer. Thus, only D-J intermediates can be PCR amplified. (C) Vδ1-Dδ2, Vδ5-Dδ2, and Dδ2-Jδ1 PCR analysis of total thymus DNA from three independent 6-wk-old E2A-deficient mice (designated a, b, and c) and their heterozygous littermates.