Motility of wild-type and amiloride-resistant strains in the presence and absence of amiloride. (A) M agar with 50 mM NaCl, 250 mM KCl, and 2.25 mM amiloride after 12 hr; (B) M agar with 300 mM NaCl after 7 hr; and (C) M agar with 75 mM LiCl after 21 hr. Expansion rates measured in the presence of amiloride were normalized to LM4303 and in the absence of amiloride were normalized to LM1017. Strains (and expansion rates in A, B, and C) are indicated. Top row, from left to right: LM4267 (0.42 ± .06, 0.94 ± .03, 0.76 ± .16), LM4268 (1.09 ± .04, 1.26 ± .07, 2.63 ± .14), and LM4601 (1.56 ± .02, 1.52 ± .08, 2.0 ± .13). Bottom row: LM4303 (1.0, 1.46 ± .13, 1.28 ± .02), LM4300 (1.64 ± .08, 1.91 ± .21, 1.29 ± .06), and LM1017 (ND, 1.0, 1.0).