Table 1.
Phenamil-resistant mutants
Strain | Parent | Isolation178 | Defective gene | Mutation |
---|---|---|---|---|
LM4467 | LM4316 | 250 mM KCl PMS | motA | D148Y |
LM4642 | LM1017 | 100 mM LiCl PMS | motA | D148Y |
LM4616 | LM1017 | 100 mM KCl PMS | motA | D148Y |
LM4641 | LM1017 | 400 mM KCl PMS | motA | D148V |
LM4620 | LM4316 | 250 mM KCl P | motA | D148G |
LM4605 | LM1017 | 250 mM KCl PMS | motA | D148G |
LM4647 | LM1017 | 200 mM KCl PMS | motA | D148G |
LM4468† | LM4316 | 250 mM KCl P | motB | A23G |
LM4613 | LM1017 | 100 mM KCl PMS | motB | A23S |
LM4615 | LM1017 | 100 mM KCl PMS | motB | A23S |
LM4621 | LM4316 | 250 mM KCl P | motB | Δ12P-16P |
LM4606 | LM1017 | 250 mM KCl PMS | motB | Δ13P-16P |
LM4603 | LM1017 | 250 mM KCl PMS | motB | G20R |
LM4604 | LM1017 | 250 mM KCl PMS | motB | G20R |
LM4644 | LM1017 | 100 mM KCl PMS | motB | G20R |
LM4645 | LM1017 | 100 mM KCl PMS | motB | G20R |
LM4646 | LM1017 | 200 mM KCl PMS | motB | G20R |
LM4614 | LM1017 | 100 mM KCl PMS | motB | L15H;G20V |
LM4470 | LM4323 | 250 mM KCl P | motB | G20V |
LM4609 | LM1017 | 250 mM KCl PMS | motB | G20V |
LM4610 | LM1017 | 250 mM KCl PMS | motB | G20V |
LM4611 | LM1017 | 250 mM KCl PMS | motB | G20V |
LM4627 | LM1017 | 250 mM KCl P | motB | G20V |
LM4469 | LM4323 | 250 mM KCl P | Not in motA, B, X, or Y | not identified |
LM4639 | LM1017 | 250 mM KCl P | Not in motA, B, X, or Y | not identified |
LM4598 | LM1017 | 250 mM KCl PMS | not in motA, B, X, or Y | not identified |
LM4649 | LM4316 | 250 mM KCl P | not in motA, B, X, or Y | not identified |
Mutants were isolated in M agar containing KCl or LiCl at the indicated concentration and 50 μM phenamil (P) or 40 μM phenamil methanesulfonate (PMS).
This strain was cured of the plasmid to make the motile strain LM4629 used in Fig. 2.