Table 2.
Classes of phenamil- and amiloride-resistant mutants
| Strain | Isolation | Amiloride178 | Phenamil178 | Rate in amiloride† | Rate in phenamil‡ | |
|---|---|---|---|---|---|---|
| Parent | LM1017 | S | S | ND§ | 0.54 ± .09 | |
| Class I | LM4609 | phenamil | S | R | ND§ | 1.0 |
| Class II | LM4598 | phenamil | R | R | 0.83 ± .09 | 1.13 ± .03 |
| LM4469 | phenamil | R | R | 1.01 ± .05 | 1.27 ± .08 | |
| LM4300 | amiloride | R | R | 1.15 ± .12 | 1.20 ± .05 | |
| LM4303 | amiloride | R | R | 1.0 | 0.93 ± .07 | |
| Class III | LM4268 | amiloride | R | S | 0.91 ± .02 | 0.57 ± .05 |
| LM4601 | amiloride | R | S | 1.13 ± .04 | 0.68 ± .08 |
178
Resistance (R) or sensitivity (S) to 2.25 mM amiloride and 40 μM phenamil.
†
Expansion rate in M agar with 300 mM NaCl and amiloride normalized to the expansion rate of LM4303.
‡
Expansion rate in M agar with 300 mM NaCl and phenamil normalized to the expansion rate of LM4609.
§
No data were collected because LM1017 and LM4609 failed to move under these conditions.