Figure 1.

Response of LACK-specific transgenic T cells to altered LACK peptides. Spleen cells (10⁶) from ABLE TCR-Cα⁰ mice were incubated with increasing amounts of the designated peptides for 48 h. Supernatants were collected from duplicate wells and assayed for IL-4 (center) and IFN-γ (right) by ELISA. Additional duplicate wells were pulsed with [³H]thymidine and incubated an additional 18 h before harvesting to assess proliferation by incorporation of radioactivity (left). No proliferation occurred in the absence of LACK peptide. Cells from TCR-Cα⁰ mice generated no signals in these assays (not shown). Results were comparable in four separate experiments. •, LACK; ▴, LACK-N164; ▪, LACK-K164; ○, OVA.