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. 1999 Apr 5;189(7):1157–1162. doi: 10.1084/jem.189.7.1157

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Cross-linking of surface CTLA-4 molecules inhibits activation and proliferation of purified CD8⁺ T cells. Resting CD8⁺ T cells were isolated from the lymph nodes of line 318 transgenic mice and cultured with sterile polystyrene beads coated with either anti-CD3 and anti–CTLA-4 or anti-CD3 and control hamster IgG. Cultures were provided either no costimulus, soluble anti-CD28, or soluble anti-CD28 and rIL-2. (A) Expression of the activation markers CD25 and CD69 on resting CD8⁺ T cells and on CD8⁺ T cells cultured in the presence (thick line) or absence (dotted line) of anti–CTLA-4. (B) ³H-TdR uptake by CD8⁺ T cells cultured in the same conditions, measured 72 h after stimulation. Results are shown as the average of triplicate wells ± SE.

Cross-linking of surface CTLA-4 molecules inhibits activation and proliferation of purified CD8⁺ T cells. Resting CD8⁺ T cells were isolated from the lymph nodes of line 318 transgenic mice and cultured with sterile polystyrene beads coated with either anti-CD3 and anti–CTLA-4 or anti-CD3 and control hamster IgG. Cultures were provided either no costimulus, soluble anti-CD28, or soluble anti-CD28 and rIL-2. (A) Expression of the activation markers CD25 and CD69 on resting CD8⁺ T cells and on CD8⁺ T cells cultured in the presence (thick line) or absence (dotted line) of anti–CTLA-4. (B) ³H-TdR uptake by CD8⁺ T cells cultured in the same conditions, measured 72 h after stimulation. Results are shown as the average of triplicate wells ± SE.