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. 1999 Apr 5;189(7):1129–1138. doi: 10.1084/jem.189.7.1129

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IRAK is dispensable for T cell development and Th1/Th2 differentiation. (A) CD4⁺ and CD8⁺ T cell populations in the thymuses and the spleens of IRAK-deficient [IRAK(−)] and wild-type mice were analyzed in flow cytometry. Immature CD4⁺ CD8⁺ double positive T cells in thymuses are also shown. (B) CD4⁺ T cells purified from the spleens and lymph nodes of IRAK-deficient [IRAK(−)] and wild-type mice were stimulated in anti-CD3 coated plates in the presence of IL-12 and anti–IL-4 for Th1 differentiation or IL-4 and anti–IFN-γ plus anti–IL-12 for Th2 differentiation. Enriched CD4⁺ T cells before (broken line) and after (solid line) 5 d of stimulation were stained with antibodies specific for CD25 and CD44 for flow cytometry analysis. (C) After 5 d of Th1 or Th2 differentiation, cells were washed and restimulated overnight with anti-CD3 antibody. IFN-γ and IL-4 levels in culture supernatants were determined by ELISA.

IRAK is dispensable for T cell development and Th1/Th2 differentiation. (A) CD4⁺ and CD8⁺ T cell populations in the thymuses and the spleens of IRAK-deficient [IRAK(−)] and wild-type mice were analyzed in flow cytometry. Immature CD4⁺ CD8⁺ double positive T cells in thymuses are also shown. (B) CD4⁺ T cells purified from the spleens and lymph nodes of IRAK-deficient [IRAK(−)] and wild-type mice were stimulated in anti-CD3 coated plates in the presence of IL-12 and anti–IL-4 for Th1 differentiation or IL-4 and anti–IFN-γ plus anti–IL-12 for Th2 differentiation. Enriched CD4⁺ T cells before (broken line) and after (solid line) 5 d of stimulation were stained with antibodies specific for CD25 and CD44 for flow cytometry analysis. (C) After 5 d of Th1 or Th2 differentiation, cells were washed and restimulated overnight with anti-CD3 antibody. IFN-γ and IL-4 levels in culture supernatants were determined by ELISA.

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