Figure 7.

Reduced MCMV-induced IFN-γ production but normal NK cell cytotoxicity in IRAK-deficient mice. IRAK-deficient [IRAK(−)] and wild-type mice were injected intraperitoneally with 10⁸ PFU of MCMV. (A) Spleen cells from mice after 71 h of infection were assayed for NK cytotoxicity against ⁵¹Cr-labeled YAC-1 target cells. (B) IFN-γ levels in sera collected at 35, 45, and 71 h of MCMV infection were determined by ELISA. (C) Expression of IFN-γ mRNA in the spleens of infected mice after 71 h of MCMV injection was determined by Northern blot hybridization using ³²P- labeled specific IFN-γ cDNA probe.