Figure 1.

α-GalCer selectively activates NK1.1⁺TCR-α/β⁺ NKT cells. Spleen cells from C57BL/6 mice were separated into a variety of lymphoid cell subsets by cell sorting as described in Materials and Methods. Their responsiveness to α-GalCer in the presence of DCs was then determined by measuring IL-4 (▪) and IFN-γ () levels in the culture supernatants using ELISA. As a control, NK1.1⁺TCR-α/β⁺ NKT cells were cultured alone or with DCs in the absence of α-GalCer. The bars represent mean ± SE of triplicate samples.