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. 1999 Apr 5;189(7):1163–1168. doi: 10.1084/jem.189.7.1163

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TCR-induced ζ phosphorylation in cultured DP thymocytes. DP thymocytes that had been cultured overnight in single cell suspension at 37°C were signaled for 5 min. DP thymocytes that have been removed from their intrathymic signaling environment and cultured overnight contain significantly increased amounts of coreceptor-associated lck (2, 3) and exhibit decreased dependence on ZAP-70 for ζ phosphorylation.