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. 1999 Apr 5;189(7):1139–1148. doi: 10.1084/jem.189.7.1139

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Analysis of CD34 and CD38 expression of highly purified CD34⁺ CD38⁻Lin⁻ cells after 6 d of culture in the presence of BMP-4. A representative experiment (n = 3) of CD34 and CD38 cell surface expression performed on initially purified CD34⁺CD38⁻Lin⁻ cells after 6 d of culture in serum-free conditions or with the addition of BMP-4 at 5 or 25 ng/ml. The entire contents of individual wells were collected at 6 d, stained with mAbs, and analyzed using flow cytometric analysis.