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. 1999 Apr 19;189(8):1329–1342. doi: 10.1084/jem.189.8.1329

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The influence of GVHD on T cell development in allogeneic chimeras. Irradiated euthymic (A and C) or thymectomized (B and D) A.BY (Thy1.2, Ly5.2) recipients received a graft containing 10⁷ T cell–depleted B6.PL (Thy1.1, Ly5.2) bone marrow cells (as a source of hematopoietic progenitors) with or without mature T cells (0.4 or 1.6 × 10⁶) harvested from the LNs of B6.SJL donors (Thy1.2, Ly5.1). Chimeras' spleen cells were analyzed by three-color staining on day 100 ± 5 posttransplant. The origin of recipient T cells was determined according to their Thy1/Ly5 phenotype. Results are presented as the mean ± SD (three to four mice per group). Note that the ordinate scale is different in A and B versus C and D. nd, not determined.