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. 1999 Apr 19;189(8):1243–1253. doi: 10.1084/jem.189.8.1243

Figure 2.

Figure 2

Figure 2

Figure 2

Expression of GrpL transcripts. Multiple tissue blots (Clontech) containing 2 μg per lane of polyA+ RNA (A) or blots containing 30 μg per lane of total RNA isolated from various hematopoietic cell lines (B) were probed with full-length GrpL. Blots were stripped and reprobed with β-actin to monitor RNA loading. (C) RT-PCR using conditions and an hGrpL primer as described in Materials and Methods was conducted on a subset of cell lines in B and additional cell lines to confirm and extend results obtained by Northern blot analysis. A primer set amplifying G3DPH was used to monitor RNA loading.