Figure 6.

GrpL regulates NF-AT activation in T cells. Jurkat T cells were transfected in triplicate with 20 μg of the NF-AT Luc and empty vector (pEF), pEF-GrpL, pEF–SLP-76, or a combination of pEF-GrpL and pEF–SLP-76 at concentrations indicated in the figure. Cells were stimulated with the anti-TCR C305 (black bars) or left unstimulated in medium (white bars). Luciferase activities were then determined on each transfected cell population. The maximal NF-AT responses for the various transfection conditions were determined by treatment with PMA plus ionomycin, and did not differ significantly. The effects of overexpressing GrpL and/or SLP-76 are shown as percentage of the maximal NF-AT responses for each transfection conditions. The data are representative of four independent experiments.