Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 1999 Apr 19;189(8):1181–1194. doi: 10.1084/jem.189.8.1181

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Biochemical characterization of gp30/40. (A) 10⁷ HPB-ALL cells lysed in Brij58-containing buffer were subjected to CD3 immunoprecipitation followed by in vitro kinase assay. 10% of the in vitro–labeled material was directly applied to two-dimensional IEF/SDS-PAGE (panels 1 and 2). The remaining 90% was subjected to reprecipitation using preimmune serum (panel 3) or immune anti-gp30/40 serum (panel 4). Panel 2 was overexposed to visualize gp30/40 in the CD3 immunoprecipitate. (B) The same experiment except that the precipitates were analyzed by one-dimensional SDS-PAGE under nonreducing (nr.) or reducing (red.) conditions. (C) Deglycosylation experiment. In vitro–labeled gp30/40 was left untreated (−) or incubated with buffer supplemented with endoglycosidase F (+) followed by SDS-PAGE and autoradiography.