Figure 3.

The cytoplasmic tail of HLA-Cw6 is not a determinant in inhibition of NK cell–mediated lysis. NK cell lines 268 (A) and 264 (B), selected by their susceptibility to inhibition by target cell expression of HLA-Cw6, were assayed for their capacity to be inhibited by target cell expression of HLA-Cw6 with truncations placed along various positions in the cytoplasmic tail. Assays were performed at various E/T cell ratios shown. Data sets shown are representative of three independent experiments for each NK line. Another independently made transfectant for HLA-Cw6/315 stop behaved similarly. A transfectant expressing HLA-Cw6 with a stop codon placed at position 332 behaved similarly to other truncated -Cw6 transfectants.