Table V.
Cytokine Production by TNBS-stimulated Cells
| Cells | Mice | TNBS enema | Stimulation with TNBS | Th1-type cytokines | Th2-type cytokines | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| IFN-γ | IL-2 | IL-4 | IL-5 | IL-6 | IL-10 | |||||||||||||
| ng/ml | U/ml | pg/ml | U/ml | ng/ml | ng/ml | |||||||||||||
| Colonic patches | IFN-γ+/+ | + | + | 0.08 ± 0.04 | 0.63 ± 0.11 | 255 ± 49 | 0.95 ± 0.20 | 6.26 ± 0.21* | 0.05 ± 0.00 | |||||||||
| + | − | <0.05 | 0.10 ± 0.01 | 24 ± 16 | 0.17 ± 0.14 | <0.1 | <0.04 | |||||||||||
| − | − | <0.05 | 0.10 | <5 | 0.10 | <0.1 | <0.04 | |||||||||||
| IFN-γ−/− | + | + | <0.05 | 1.02 ± 0.25 | 114 ± 24 | 2.68 ± 1.00 | 0.66 ± 0.18* | 0.32 ± 0.25 | ||||||||||
| + | − | <0.05 | 0.42 ± 0.25 | 5 ± 4 | 0.47 ± 0.34 | 0.55 ± 0.08 | <0.04 | |||||||||||
| − | − | <0.05 | <0.05 | 5 | 0.65 | <0.1 | 0.1 | |||||||||||
| IFN-γ+/+ | + | + | 0.30 ± 0.11 | 1.62 ± 0.19 | 215 ± 41 | 1.15 ± 0.36* | 0.25 ± 0.03 | 0.20 ± 0.13 | ||||||||||
| + | − | 0.06 ± 0.04 | 0.58 ± 0.25 | <5 | <0.1 | 0.01 ± 0.01 | 0.17 ± 0.00 | |||||||||||
| SLN | − | − | 0.07 | <0.05 | <5 | <0.1 | <0.1 | <0.04 | ||||||||||
| + | + | <0.05 | 1.43 ± 0.11 | 174 ± 14 | 3.38 ± 0.42* | 0.19 ± 0.10 | 0.32 ± 0.25 | |||||||||||
| IFN-γ−/− | + | − | <0.05 | 0.31 ± 0.25 | 2 ± 2 | 0.46 ± 0.23 | 0.11 ± 0.10 | <0.04 | ||||||||||
| − | − | <0.05 | <0.05 | <5 | <0.1 | <0.1 | <0.04 | |||||||||||
Values shown are the average ± 1 SD of three independently prepared cell fractions. Values of mice without TNBS enema were obtained from a pooled cell fraction taken from 10 mice. TNBS specific cytokine responses were not observed for IL-6 in IFN-γ−/− mice and for IL-10 in SLN of IFN-γ+/+ mice. In other studies, lamina propria lymphocytes from IFN-γ−/− and IFN-γ+/+ mice were assessed for Th1 and Th2 cytokines and in all instances, neither Th1- nor Th2-type cytokines were detected.
*
P < 0.02.