Figure 1.

TRAIL, FasL, and TNF expression on human Mφ. (A) Mφ were incubated for 2 or 12 h in the absence or presence of IFN-γ, IFN-α, GM-CSF, or LPS and then analyzed for TRAIL or FasL surface expression. Filled histograms represent staining at 12 h by either M181 (anti-TRAIL mAb) or NOK-1 (anti-FasL mAb). Open histograms represent staining at 2 h with the same mAb, whereas dotted histograms represent staining with isotype control mAb. (B) Mφ were incubated as in A and then analyzed for TNF surface expression. Filled histograms represent staining at 2 h by mAb11 (anti-TNF mAb). Open histograms represent staining at 12 h with the same mAb, whereas dotted histograms represent staining with isotype control mAb. Histograms represent 10⁴ gated Mφ in all conditions, and viability was >95% as assessed by propidium iodide exclusion. These observations were reproduced using Mφ from at least five different donors.