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. 1999 Mar 15;189(6):931–938. doi: 10.1084/jem.189.6.931

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Representative autoradiograph of a polyacrylamide gel used in typical ribonuclease protection assay comparing PSGL-1 and P-selectin mRNA expression in lungs of sham-operated control and hemorrhaged wild-type mice. Compared with lanes containing PSGL-1, mRNA levels from sham-operated wild-type mice (lane 1) there is a marked increase in PSGL-1 mRNA in lung isolated from wild-type mice subjected to hemorrhagic shock (lane 2). No significant difference was observed in P-selectin mRNA levels between the two groups of mice. Densitometric quantification of the effect of hemorrhage on lung PSGL-1 mRNA expression is summarized in the right panel of the figure. Bar heights represent means and brackets indicate ± SEM. Numbers at the bottom of each bar represent the number of mice studied.