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. 1999 Mar 15;189(6):907–918. doi: 10.1084/jem.189.6.907

Figure 3.

Figure 3

Effect of heat and proteinases on PSM activity. (A) PSM at 10 μg/ml in RPMI was heated in a boiling water bath for the periods indicated. For determination of luciferase synthesis, the reaction mixtures contained 2 × 106 THP-1 LTRluc, either unheated or heated PSM at 100 ng/ml, and RPMI at a final volume of 2.0 ml. The results are the mean (− background) ± SEM of four experiments. The asterisk indicates a significant difference from unheated PSM. (B) PSM was preincubated with immobilized proteinase (proteinase K, Streptomyces griseus protease, trypsin) as described under Materials and Methods and activation of the LTR compared with that produced by untreated PSM or PSM preincubated with agarose beads without bound proteinase. The results are the mean ± SEM of three experiments. The asterisk indicates a significant difference from PSM alone (P < 0.05, unpaired Student's t test).