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. 1999 Mar 15;189(6):979–990. doi: 10.1084/jem.189.6.979

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Immunofluorescence profiles of the 4C8 antigen on human PBLs and HUVECs. PBMC and neutrophils from a healthy donor were stained with FITC-conjugated anti-4C8 and phycoerythrin-Cy5–conjugated anti-CD3 mAb or control FITC-conjugated IgG3. The stained leukocytes as well as HUVECs (10,000 cells) were analyzed by a FACScan^® (Becton Dickinson) flow cytometer with gating on the lymphocyte, monocyte, and neutrophil populations.