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. 1999 Mar 15;189(6):979–990. doi: 10.1084/jem.189.6.979

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Soluble anti-4C8 mAb augments F-actin content in CD26^hi T cells. T cells were incubated for 3 h with anti-4C8 or control IgG3. The cells were fixed, permeabilized, and stained with FITC-conjugated phalloidin and rhodamine-conjugated anti-CD26 mAb. The stained cells were analyzed by a flow cytometer. Increased F-actin content was observed in CD26^hi T cells at 10 μg/ml, but not 1 μg/ml, of anti-4C8. The staining shown is representative of three independent experiments.