Figure 2.

Western blot analysis of the 4C8 antigen. SDS–gel electrophoresis of lysates from PBMC and neutrophils (5.5 × 10⁶ cells/lane) was performed under reducing conditions. DFP, a serine protease inhibitor, was used to prevent proteolytic activity in the neutrophil lysates. Control Ab was purified mouse IgG3 of the same isotype as anti-4C8. See Materials and Methods for details.