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. 1999 Mar 15;189(6):979–990. doi: 10.1084/jem.189.6.979

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Anti-4C8 mAb inhibits T cell migration through, but not adhesion to, HUVEC monolayers. Freshly isolated CD3⁺ T cells were incubated with unstimulated and IFN-γ–stimulated (500 U/ml, 48 h) HUVEC monolayers cultured on collagen gels in the continuous presence of anti-4C8 (1 μg/ml), anti-CD11a (10 μg/ml), or control IgG3 (10 μg/ml). The numbers of adherent cells and migrated cells were determined as described in Materials and Methods. Results of T cell adhesion (A) and migration (B) are expressed as the adhesion and the migration index, respectively, and represent the mean ± SD of five independent experiments. The index (%) is calculated as follows: the number of adherent or migrated T cells with antibody/the number of adherent or migrated T cells without antibody × 100. The numbers of the background adhesion and migration with unstimulated HUVEC are 376 ± 84 and 131 ± 4, respectively.

Anti-4C8 mAb inhibits T cell migration through, but not adhesion to, HUVEC monolayers. Freshly isolated CD3⁺ T cells were incubated with unstimulated and IFN-γ–stimulated (500 U/ml, 48 h) HUVEC monolayers cultured on collagen gels in the continuous presence of anti-4C8 (1 μg/ml), anti-CD11a (10 μg/ml), or control IgG3 (10 μg/ml). The numbers of adherent cells and migrated cells were determined as described in Materials and Methods. Results of T cell adhesion (A) and migration (B) are expressed as the adhesion and the migration index, respectively, and represent the mean ± SD of five independent experiments. The index (%) is calculated as follows: the number of adherent or migrated T cells with antibody/the number of adherent or migrated T cells without antibody × 100. The numbers of the background adhesion and migration with unstimulated HUVEC are 376 ± 84 and 131 ± 4, respectively.