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Anti-4C8 mAb blocks transmigration of T cells at the junctional level. T cells were incubated for 4 h on an IFN-γ–stimulated HUVEC monolayer in the presence of anti-4C8 (1 μg/ml). The culture was washed, fixed, and prepared for scanning electron microscopy. Tightly adherent cells extending pseudopods into the junction could be seen. Bar, 5 μm.
