Figure 8.

The profile of CD26 expression on T cells that migrated through HUVEC monolayers (A) and into collagen gels with impregnated anti-4C8 mAb (B). (A) After T cells were incubated for 5 h with resting HUVEC monolayers, unbound, adherent (but not migrating), and migrated T cells were isolated as described in Materials and Methods. (B) T cells were incubated for 5 h on collagen gels with impregnated anti-4C8 (10 μg/ml) or control IgG3 (10 μg/ml). After unbound cells and cells adhering to the apical surface of collagen gels were removed with EDTA treatment, migrated cells were released from the gels by treatment with collagenase. The isolated cells were stained with fluorescein-conjugated anti-CD26 and anti-CD3 mAbs. The CD26 expression of the cells was analyzed by flow cytometry with gating on CD3⁺ cells.