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. 1999 Mar 15;189(6):979–990. doi: 10.1084/jem.189.6.979

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Morphologic changes of T cells and redistribution of F-actin induced by immobilized anti-4C8 mAb. T cells were incubated for 2 h on glass slides precoated with anti-4C8 (10 μg/ml) or control IgG3 (10 μg/ml). After staining with TRITC-conjugated phalloidin, microscopic observation was performed (×1,000). Extreme cell polarization and large pseudopods rich in F-actin were noted with immobilized anti-4C8 (B) but not IgG3 (A).

Morphologic changes of T cells and redistribution of F-actin induced by immobilized anti-4C8 mAb. T cells were incubated for 2 h on glass slides precoated with anti-4C8 (10 μg/ml) or control IgG3 (10 μg/ml). After staining with TRITC-conjugated phalloidin, microscopic observation was performed (×1,000). Extreme cell polarization and large pseudopods rich in F-actin were noted with immobilized anti-4C8 (B) but not IgG3 (A).