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. 1999 May 3;189(9):1507–1512. doi: 10.1084/jem.189.9.1507

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Competitive inhibition of IgE binding to solid phase–coated A. fumigatus recombinant P₂ protein. Serum from A. fumigatus–sensitized patients was preincubated with increasing amounts of recombinant A. fumigatus (○), human P₂ protein (•), or Asp f 1 as negative control (□). Preincubated serum samples were transferred to wells coated with A. fumigatus P₂ protein, and bound IgE was analyzed by antigen-specific ELISA (8, 16).