Figure 4.
Skin test reactivity to recombinant A. fumigatus and human proteins in a patient sensitized to MnSOD and P2 protein. For intradermal skin tests, 100 μl of the protein solutions (10−1 μg/ml) was injected with a syringe. 0.01% histamine dihydrochloride (A) and 0.9% saline (B) were used as positive and negative controls, respectively. The reactions show that 10 ng fungal (D) and human (E) P2 protein or fungal (H) and human (I) MnSOD are able to elicit a wheal that is comparable to the size of the skin reaction induced by the positive histamine control. C shows the reaction to a challenge with 1 ng human P2 protein. The patient lacks IgE to rAsp f 3 (F) and rAsp f 11 (G), two additional A. fumigatus allergens (10). The absence of reactions to skin challenges with these allergens demonstrates the specificity of the test.