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. 1999 May 3;189(9):1497–1506. doi: 10.1084/jem.189.9.1497

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(A and B) Transfection of COS cells with a cDNA clone (isolated by an expression cloning strategy) confers PAL-1 reactivity. COS cells were transfected with the isolated cDNA clone (A) or the empty vector (B); cytospins prepared 48 h later, and they were immunostained with mAb PAL-1. Control IgM antibody showed no labeling (not shown). (C) Transfection of COS cells with MARCO cDNA (for sequence identification, see Fig. 6) confers mAb PAL-1 inhibitable TiO₂ binding. COS cells, transfected with MARCO cDNA, were pretreated with mAb PAL-1 and a control mAb and their binding of TiO₂ determined using a flow cytometric assay. The data represent mean (± SEM) RAS increase of five separate experiments. *Significantly different from TiO₂ alone, P < 0.01.

(A and B) Transfection of COS cells with a cDNA clone (isolated by an expression cloning strategy) confers PAL-1 reactivity. COS cells were transfected with the isolated cDNA clone (A) or the empty vector (B); cytospins prepared 48 h later, and they were immunostained with mAb PAL-1. Control IgM antibody showed no labeling (not shown). (C) Transfection of COS cells with MARCO cDNA (for sequence identification, see Fig. 6) confers mAb PAL-1 inhibitable TiO₂ binding. COS cells, transfected with MARCO cDNA, were pretreated with mAb PAL-1 and a control mAb and their binding of TiO₂ determined using a flow cytometric assay. The data represent mean (± SEM) RAS increase of five separate experiments. *Significantly different from TiO₂ alone, P < 0.01.

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