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. 1999 May 3;189(9):1497–1506. doi: 10.1084/jem.189.9.1497

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(A–D) Human AMs express MARCO. Immunostaining of normal volunteer BAL cytocentrifuge preparations (A) and surgical lung tissue specimens (C) showed strong labeling by a rabbit anti–human MARCO antibody; control rabbit IgG showed only minimal nonspecific background staining (B, D) (original magnification 400). (E) Lysates prepared from BAL cells collected from adult volunteers (2/5 similar results shown) were analyzed by reducing SDS-PAGE and immunoblotted with a rabbit anti–human MARCO antibody or control rabbit IgG (Ctrl). Relative molecular mass standards are indicated (kD).

(A–D) Human AMs express MARCO. Immunostaining of normal volunteer BAL cytocentrifuge preparations (A) and surgical lung tissue specimens (C) showed strong labeling by a rabbit anti–human MARCO antibody; control rabbit IgG showed only minimal nonspecific background staining (B, D) (original magnification 400). (E) Lysates prepared from BAL cells collected from adult volunteers (2/5 similar results shown) were analyzed by reducing SDS-PAGE and immunoblotted with a rabbit anti–human MARCO antibody or control rabbit IgG (Ctrl). Relative molecular mass standards are indicated (kD).

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