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. 1999 Jun 7;189(11):1715–1722. doi: 10.1084/jem.189.11.1715

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Specificity of the bead rosetting assays. COS-1 cells were cotransfected with the indicated FcR construct together with pCMV-GFP. GFP⁺ cells were purified as described in Materials and Methods and incubated with the relevant murine IgM-blocking mAb or an irrelevant murine IgM control mAb for 30 min before addition of Ig-coated beads. Results are shown as percentage of inhibition of rosette formation when compared with transfectants that were not incubated with mAb before rosetting analysis. Results shown are representative of two experiments.