Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 1999 Jun 7;189(11):1715–1722. doi: 10.1084/jem.189.11.1715

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

(A) FACS^® analysis of newly produced mAbs to FcαRI compared with mAb A77 of similar specificity. FcαRI-expressing murine B cells (IIA1.6) were incubated with mAbs as indicated (white peaks) or with concentration and isotype-matched control mAbs (hatched peaks), followed by a GAM IgG1–PE conjugate. (B) FcαRI-expressing IIA1.6 cells were incubated without (left panel) or with mAbs as indicated, followed by heat-aggregated hIgA (aIgA). After 1 h at 4°C, cells were washed and bound hIgA was detected by incubation with goat anti–human IgA F(ab)₂-PE conjugate (white peaks). Cells incubated with only the secondary reagent served as negative controls (hatched peaks). (C) Reactivity of chimeras (left) with a panel of FcαR and bFcγ2R mAb (top) as measured by FACS^® analysis. Binding was graded as follows: +, strong binding; +/−, weak binding; −, no binding.

(A) FACS^® analysis of newly produced mAbs to FcαRI compared with mAb A77 of similar specificity. FcαRI-expressing murine B cells (IIA1.6) were incubated with mAbs as indicated (white peaks) or with concentration and isotype-matched control mAbs (hatched peaks), followed by a GAM IgG1–PE conjugate. (B) FcαRI-expressing IIA1.6 cells were incubated without (left panel) or with mAbs as indicated, followed by heat-aggregated hIgA (aIgA). After 1 h at 4°C, cells were washed and bound hIgA was detected by incubation with goat anti–human IgA F(ab)₂-PE conjugate (white peaks). Cells incubated with only the secondary reagent served as negative controls (hatched peaks). (C) Reactivity of chimeras (left) with a panel of FcαR and bFcγ2R mAb (top) as measured by FACS^® analysis. Binding was graded as follows: +, strong binding; +/−, weak binding; −, no binding.