Figure 1.

Antigenicity of synthetic peptides corresponding to dominant and subdominant determinants. (A) T2-K^d cells were cultured for 14 h at 26°C and added to the wells containing synthetic peptides at the indicated concentrations. The samples were immediately shifted to 37°C and incubated for 2 h to denature K^d molecules lacking peptides and then stained with a fluorescein-conjugated anti-K^d mAb. The mean channel fluorescence (MCF) of viable cells was determined by flow cytometry. (B) Splenocytes from PR8-primed animals stimulated in vitro for 7 d with synthetic peptides corresponding to NP_39–47, NP_147–155, or NP_218–226 were tested in a microcytotoxicity assay for their ability to lyse ⁵¹Cr- labeled P815 target cells incubated in I-10 with synthetic peptides at the indicated concentrations.