Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 1999 Jun 7;189(11):1757–1764. doi: 10.1084/jem.189.11.1757

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

RP-HPLC mass spectrometric characterization of synthetic NP_218–226. 10 μmol of NP_218–226, either a freshly made stock in PBS (A) or 1-yr stock in DMSO (D), were added to 300 μl of cystine-free or cystine-containing DME, incubated for 2 h at room temperature, and analyzed by RP-HPLC. The masses of material in eluting peaks were determined by MALDI-TOF, revealing that cysteinylated NP_218–226 elutes at 25 min, unmodified NP_218–226 at 27 min, and dimeric NP_218–226 at 29 min. A, in PBS + TCEP; B, monomer in cystine-free medium; C, monomer in cystine-containing medium; D, in DMSO; E, dimer in cystine-free medium; F, dimer in cystine-containing medium.