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. 1999 Jun 7;189(11):1757–1764. doi: 10.1084/jem.189.11.1757

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Cysteinylated NP_218–226 is presented in vivo. T_CD8+ were produced from PR8-infected mice by stimulation with HPLC-purified, cysteinylated (A) or unmodified (B) NP_218–226. Synthetic NP_218–226 was cysteinylated by dilution into serum-free DME and incubation for 2 h at room temperature or maintained in unmodified form by addition of 200 μM TCEP. Diluted peptides were then added into assay wells containing T_CD8+ and ⁵¹Cr-labeled P815 targets, and lysis was determined by microcytotoxicity assay.